Chromosome pairing and recombination during meiosis in Caenorhabditis elegans

Links between DNA damage and hydroxymethylcytosine in mammalian cells

meiotic cells of c. elegans viewed with superresolution 3d structured illumination microscopy

meiotic cells of c. elegans viewed with superresolution 3d structured illumination microscopy

Meiosis is an essential cell division process that creates haploid cells (e.g., sperm and eggs) from diploid precursors. Errors in meiosis cause many human reproductive problems, from infertility to birth defects. Using the nematode Caenorhabditis elegans as a model system for its excellent genetic and cytological qualities, we aim to find the mechanisms underlying correct pairing and synapsis of homologous chromosomes, as well as regulation of recombination.

減数分裂生物学:減数分裂は、二倍体の前駆細胞から、精子や卵子などの一倍体生殖細胞を作り出す必須の細胞分裂です。減数分裂におけるエラーは、人間の場合、不妊問題や染色体異常の新生児などの生殖問題に繋がります。私たちは、卵母細胞が非常に豊富なモデル生物、線虫を用いて、減数分裂の前期、どのように母方と父方の相同染色体が出会い、ペアになり、そして減数第一分裂で正常に分離されるのか、という謎にアプローチしています。減数分裂のメカニズムの理解し、私たちの社会が直面する生殖問題の理解に貢献することを目指します。

HeLa cell showing colocalization of  5hmc (red) and DNA DAMAGE PROTEIN 53BP1  (green)

HeLa cell showing colocalization of  5hmc (red) and DNA DAMAGE PROTEIN 53BP1  (green)

DNA is always under attack from hazards in our environment such as cancerous chemicals and the UV in sunlight, but our cells are constantly repairing the damage to keep our DNA in good condition. We have found that regions of damaged DNA become marked with the chemical tag 5-hydroxymethylcytosine, and that cells unable to make this chemical modification have problems with cell division after DNA damage. We are researching the role of 5-hydroxymethylcytosine in the DNA damage and repair pathways in mammalian cells.

哺乳類細胞を用いて、DNA修復時に、DNA上に起こる修飾5-ヒドロキシメチルシトシン(5hmC)の役割を明らかにしようとしています。